Please use this identifier to cite or link to this item:
https://doi.org/10.48548/pubdata-1478
| Resource type | Journal Article |
| Title(s) | Microbial nutrient limitation and catalytic adjustments revealed from a long-term nutrient restriction experiment |
| DOI | 10.48548/pubdata-1478 |
| Handle | 20.500.14123/1552 |
| Creator | Kumar, Amit  0000-0003-4590-1825Pausch, Johanna 0000-0002-7102-4793 |
| Abstract | Introduction: Microbial abundance and activities in soils are predominantly determined by soil carbon (C), nitrogen (N) and phosphorus (P) availability. Much research has focused on the effects of soil N than P availability on soil microbial biomass and enzyme activities as sensitive proxies of microbial ecophysiology highlighting the need to investigate how microbes will respond to P availability in soil, especially in cropping systems. Materials and Methods: The effect of P fertilisation on microbial biomass-C, -N and -P, and the kinetic parameters (maximal velocity [Vmax], Michaelis constant [Km] and catalytic efficiency [Ka]) of β-1,4-glucosidase (BG; C-acquiring), leucine-aminopeptidase (LAP; predominantly N-acquiring) and acid phosphomonoesterase (PHO; P-acquiring) were measured in a nutrient-poor agricultural soil (devoid of fertiliser application since 1942). Results: This study showed that P fertilisation led to a 65% and 56% increase in microbial biomass-N and -P, respectively, indicating severe P limitation and inefficient N acquisition by microbes without P availability. Increased Ka values of LAP with P fertilisation further hint toward the production of efficient isoenzymes to avoid resource tradeoffs for nutrient acquisition. Conclusions: Overall, these results decipher microbial metabolic and catalytic adjustments mediated by soil P availability. Increased microbial biomass-N and -P with P fertilisation indicated microbial N and P colimitation that was partly overcome by the production of efficient enzymes for N acquisition with P fertilisation. We argue to incorporate microbial enzyme activities as a response to different management strategies to better inform us about soil biogeochemical cycles in cropping systems. |
| Language | English |
| Keywords | Catalytic Efficiency; Enzyme Activity; Kinetic Parameters; Microbial Biomass; Phosphorus; Fertilization |
| Year of publication in PubData | 2024 |
| Publishing type | Parallel publication |
| Publication version | Published version |
| Date issued | 2022-04-11 |
| Creation context | Research |
| Notes | This publication was funded by the Open Access Publication Fund of Leuphana University Lüneburg. |
| Published by | Medien- und Informationszentrum, Leuphana Universität Lüneburg |
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open-access | 1.35 MB | Adobe PDF | View/Open |
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